The 21st century is marked by a triple challenge: meeting the nutritional needs of a rapidly growing global population, addressing climate change, and creating a more sustainable and progressive agricultural model. Against this backdrop, Precision Plant Breeding (PPB) emerges as a pioneering and innovative solution that leverages advances in genome editing to target and efficiently improve the quality and yield of tomatoes. The Zinc Finger Nucleases (ZFNs) technology offers the ability to modify the genome with precision, enabling the creation of new, elite varieties in short time and high-throughput manner without introducing foreign genetic material. The selected gene target is a critical transcription factor (NF-YA) involved in plant development, tomato quality, and characteristics, influencing multiple genes. As a cutting-edge technology, PPB holds promise for advancing crop improvement across various plant species, contributing to the addressing of challenges related to food security, sustainable agriculture, and climate change.
